|
KMID : 1007520120210041149
|
|
Food Science and Biotechnology
2012 Volume.21 No. 4 p.1149 ~ p.1155
|
|
|
Bioactive Compounds Extracted from Gamtae (Ecklonia cava) by Using Enzymatic Hydrolysis, a Potent α-Glucosidase and α-Amylase Inhibitor, Alleviates Postprandial Hyperglycemia in Diabetic Mice
|
|
Lee Seung-Hong
Park Mi-Hwa
Han Ji-Sook
Jeong Yoon-Hwa
Kim Mi-Sook
Jeon You-Jin
|
|
|
Abstract
|
|
|
|
This study was designed to investigate whether the brown alga gamtae (Ecklonia cava) may inhibit α- glucosidase and α-amylase activities, and alleviate postprandial hyperglycemia in streptozotocin-induced diabetic mice. For that purpose, we prepared an enzymatic hydrolysate from gamtae (EHG) by using the carbohydrase, Celluclast. EHG evidenced prominent inhibitory effect against α- glucosidase and α-amylase. The IC50 values of EHG against α-glucosidase and α-amylase were 0.62 and 0.59 mg/mL, respectively, which evidenced the higher activities than that of acarbose. EHG did not exert any cytotoxic effect in human umbilical vein endothelial cells (HUVECs) at various concentrations (from 0.25 to 2 mg/mL). The increase of postprandial blood glucose levels were significantly suppressed in the EHG administered group than those in the streptozotocin-induced diabetic or normal mice. Moreover, the area under curve (AUC) was significantly reduced via EHG administration (6,102 vs. 10,425 mg·min/dL) in the diabetic mice as well as it delays absorption of dietary
|
|
|
KEYWORD
|
|
|
gamtae, celluclast, α-glucosidase, α-amylase, hyperglycemia
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|